Journal: Microscopy and Microanalysis

Article Title: Robot-Guided Atomic Force Microscopy for Mechano-Visual Phenotyping of Cancer Specimens

doi: 10.1017/s1431927615015007

Figure Lengend Snippet: Figure 1. Unstained tissue imaged with atomic force microscope (AFM). a: 4 × view (without probe) of a tissue section, showing the limited field of view presented by hardware configuration of the AFM. b: 10× view of a 0.6 mm tissue microarray disc with AFM probe.

Article Snippet: The AFM experimental setup, as shown in Figures 6a and 6b, consists of an AFM (MFP-3D-BIOTM; Asylum Research, an Oxford Instruments Company, Santa Barbara, CA, USA) integrated with an inverted optical microscope (Nikon TE2000U; Nikon Instruments Inc., Melville, NY, USA) and fitted with a customized robotic stage driven by a stepper motor-controlled micromanipulator (MP-285; Sutter Instruments, Novato, CA, USA), which is placed on the vibration isolation table.

Techniques: Microscopy, Microarray

Journal: Microscopy and Microanalysis

Article Title: Robot-Guided Atomic Force Microscopy for Mechano-Visual Phenotyping of Cancer Specimens

doi: 10.1017/s1431927615015007

Figure Lengend Snippet: Figure 6. Atomic force microscopic (AFM) experimental setup. a, the glass slide with TMA placed on the XY-stage; b, the AFM head attached with AFM probe placed over the glass slide for tissue indentation; c, the magnified view of AFM probe aligned at the start point of the ROI in the tissue. Illustrated by circled numbers: (1) AFM charge-coupled device camera, (2) AFM inverted microscope, (3) vibration isolation table, (4) MP-285 micromanipulator, (5) tissue slide, (6) slide holder, (7) AFM head, (8) AFM probe, (9) tissue, (10) probing region of interest (ROI), and (11) tracking ROI.

Article Snippet: The AFM experimental setup, as shown in Figures 6a and 6b, consists of an AFM (MFP-3D-BIOTM; Asylum Research, an Oxford Instruments Company, Santa Barbara, CA, USA) integrated with an inverted optical microscope (Nikon TE2000U; Nikon Instruments Inc., Melville, NY, USA) and fitted with a customized robotic stage driven by a stepper motor-controlled micromanipulator (MP-285; Sutter Instruments, Novato, CA, USA), which is placed on the vibration isolation table.

Techniques: Inverted Microscopy, Isolation

Journal: Microscopy and Microanalysis

Article Title: Robot-Guided Atomic Force Microscopy for Mechano-Visual Phenotyping of Cancer Specimens

doi: 10.1017/s1431927615015007

Figure Lengend Snippet: Figure 9. An example procedure of registering panels of images based on known landmarks of the specimen. Left: simulated specimen on atomic force microscope. Middle: hematoxylin and eosin image. Right: P63/smooth muscle actin double stain.

Article Snippet: The AFM experimental setup, as shown in Figures 6a and 6b, consists of an AFM (MFP-3D-BIOTM; Asylum Research, an Oxford Instruments Company, Santa Barbara, CA, USA) integrated with an inverted optical microscope (Nikon TE2000U; Nikon Instruments Inc., Melville, NY, USA) and fitted with a customized robotic stage driven by a stepper motor-controlled micromanipulator (MP-285; Sutter Instruments, Novato, CA, USA), which is placed on the vibration isolation table.

Techniques: Microscopy, Staining

Journal: Microscopy and Microanalysis

Article Title: Robot-Guided Atomic Force Microscopy for Mechano-Visual Phenotyping of Cancer Specimens

doi: 10.1017/s1431927615015007

Figure Lengend Snippet: Figure 10. Example procedure of sampling a morphologically interesting feature. a: Overlaying a hand-drawn necrotic region to simulated atomic force microscopic (AFM) specimen to guide navigation of the AFM microscope to general location of the interested feature. b: Smooth muscle actin signal on the right panel guided the selection of the region- of-interest, which was illustrated in black on all panels. c: Two-dimensional elastic modulus map of the scan region showing the distinction of elastic features between soft necrotic tissue and rigid desmoplastic region, although the epi- thelial region in between is not as prominent. The color at each sampling point indicates the elastic modulus computed from the AFM curve at the location.

Article Snippet: The AFM experimental setup, as shown in Figures 6a and 6b, consists of an AFM (MFP-3D-BIOTM; Asylum Research, an Oxford Instruments Company, Santa Barbara, CA, USA) integrated with an inverted optical microscope (Nikon TE2000U; Nikon Instruments Inc., Melville, NY, USA) and fitted with a customized robotic stage driven by a stepper motor-controlled micromanipulator (MP-285; Sutter Instruments, Novato, CA, USA), which is placed on the vibration isolation table.

Techniques: Sampling, Microscopy, Selection